ABSTRACT
Objective@#To investigate current status of screen-time in 2-6 years old preschoolers in Beijing, and to describe associated factors of high screen time, and to provide a reference for making interventional measures to prevent and control short-sightness.@*Methods@#A total of 366 preschoolers were chosen through convenience sampling from 5 kindergartens in Beijing urban and rural areas. Questionnaire survey was administered to parents regarding child screen-time.@*Results@#The average screen time of the preschool children in Beijing was (120.5±78.5)min/d, and the rate of high screen time was 75.8%. The average screen time during weekdays was (92.4±72.8) min/d, which was less than the average screen time at weekends (192.4±117.0) min/d. Logistic regression analysis showed that age (OR=1.69, 95%CI=1.19-2.38, P＜0.05) and without an habit of exercise among parents (OR=3.05, 95%CI=1.50-6.19, P＜0.05) were positively associated with, and being girl was the negatively associated with high screen-time (OR=0.49, 95%CI=0.25-0.99, P＜0.05).@*Conclusion@#A large proportion of the preschool children aged 2-6 in Beijing have too long screen time, especailly during weekend, and gender, age, parental habit of exercising and so on have impacts on their screen time. Therefore, the education and prevention work should be conducted to reduce their screen time.
ABSTRACT
@#[Abstract] Objective: To explore the inhibitive effect of asiatic acid (AA) on paclitaxel (PTX)-resistant glioma cells and its possible mechanism. Methods: The effects of AA on the proliferation and apoptosis of glioblastoma U87MG cells were detected by CCK-8 assay, Real-time quantitative polymerase chain reaction (qPCR) and Western blotting. The drug-resistant glioma cell line PR-U87MG was established by culturing the cells in concentration-increasing PTX. With U87MG cells as control, the PTX-resistance of PR-U87MG cells was confirmed using CCK-8 assay, and the mRNA and protein levels of MDR1 and LRP were measured with qPCR and western blotting. PR-U87MG cells were treated with AA, PTX or AA+PTX, and then the cell viability and apoptosis of each group were measured with CCK-8 assay, qPCR and Western blotting. Results: PTX-resistant PR-U87MG cell line was successfully established. AA inhibited the viability of U87MG and PR-U87MG cells in a dose-dependent manner (P<0.01) and significantly promoted their apoptosis (P<0.01). Compared with the group treated with AA or PTX alone, the group treated with the combination of AA and PTX had significantly decreased protein levels of PARP1 (P<0.01), drug-resistant related proteins (Pgp-1 and LRP [lung resistance protein], all P< 0.01), and markedly increased caspase 3 (P<0.01). Conclusion: AA could effectively enhance the sensitivity of U87MG cells to PTX, and the mechanism may be related to the suppressed expression of drug efflux-associated proteins Pgp-1 and LRP.